Determination of creatinine in human urine by ion chromatography with an immobilized creatinine deiminase reactor.
نویسندگان
چکیده
منابع مشابه
Simultaneous determination of creatinine and uric acid in human urine by high-performance liquid chromatography.
An environmentally friendly reversed-phase HPLC method for simultaneous determination of creatinine and uric acid in human urine samples has been developed. Human urine samples were pretreated by dilution, protein precipitation, centrifugation and filtration, followed by HPLC separations using a reversed-phase C(18) column with an aqueous mobile phase of phosphate buffer. The retention loss of ...
متن کاملDetermination of creatinine in serum and urine by cation-exchange high-pressure liquid chromatography.
We describe an HPLC method for quantifying creatinine, separating the analyte from other compounds in serum and urine by cation-exchange chromatography and measuring its absorbance at 234 nm. The precision of the method (CV) varied from 2.9% (mean creatinine concentration, 31 mumol/L) to 1.7% (361 mumol/L) within a series of assays and from 3.9% (34 mumol/L) to 2.4% (391 mumol/L) between series...
متن کاملCreatinine Determination in Urine by Liquid Chromatography-electrospray Ionization-tandem Mass Spectrometry Method.
Creatinine determination in urine is used to estimate the completeness of the 24-h urine collection, compensation for variable diuresis and as a preliminary step in protein profiling in urine. Despite the fact that a wide range of methods of measuring creatinine level in biofluids has been developed, many of them are adversely affected by interfering substances. A new liquid chromatography-tand...
متن کاملOn the Determination of Creatinine and Creatine in Urine
Ever since the introduction of the calorimetric method for the determination of creatinine, potassium bichromate, in half normal solution, has been used as the standard for measuring the color obtained in the reaction of creatinine with picric acid and sodium hydroxide. That the bichromate has been serviceable for the purpose is evidenced by the fact that no other standard has ever been propose...
متن کاملOn the Determination of Creatinine and Creatine in Urine
Ever since the introduction of the calorimetric method for the determination of creatinine, potassium bichromate, in half normal solution, has been used as the standard for measuring the color obtained in the reaction of creatinine with picric acid and sodium hydroxide. That the bichromate has been serviceable for the purpose is evidenced by the fact that no other standard has ever been propose...
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 1988
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.4.423